Hotspot

ycf3-trnS-GGA

LSC · 44462-45205 · 744 bp

Back to Ceratocephala

Pi 0.0257

MarkerSeek score 65.10

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 744
variable_sites: 19
pi: 0.0257
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CCATTTATCCGTGATCTAGGCA	TCGGTAAACAAAAGCCCACA	45.5	45.0	57.7	57.9	831 bp	1.0	63.8
ycf3-trnS-GGA_p2	CCATTTATCCGTGATCTAGGCA	GCGAAGGTACGGAAAGAGAG	45.5	55.0	57.7	58.1	863 bp	1.0	63.4
ycf3-trnS-GGA_p3	CCATTTATCCGTGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	45.5	50.0	57.7	58.2	855 bp	1.0	63.0
ycf3-trnS-GGA_p4	CCATTTATCCGTGATCTAGGCA	AAAGCCCACATAGCAGTTCC	45.5	50.0	57.7	58.2	821 bp	1.0	63.0
ycf3-trnS-GGA_p5	CCATTTATCCGTGATCTAGGCA	TAGGCGAAGGTACGGAAAGA	45.5	50.0	57.7	58.2	866 bp	1.0	62.8