Hotspot

petN-psbM

LSC · 28159-29374 · 1216 bp

Back to Ceratocephala

Pi 0.00164

MarkerSeek score 59.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1216
variable_sites: 2
pi: 0.00164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	CATTCATTGCTACTGCGCTG	45.0	50.0	58.0	57.9	1378-1382 bp	1.0	79.3
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	ATTCATTGCTACTGCGCTGT	45.0	45.0	58.0	58.3	1377-1381 bp	1.0	78.0
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	ATTGCTACTGCGCTGTTCAT	45.0	45.0	58.0	58.3	1373-1377 bp	1.0	78.0
petN-psbM_p4	AGTAAGTCTTGCTTGGGCTG	CATTCATTGCTACTGCGCTG	50.0	50.0	57.8	57.9	1384-1388 bp	1.0	77.8
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	ATTGCTACTGCGCTGTTCAT	50.0	45.0	57.8	58.3	1379-1383 bp	1.0	76.5