Hotspot

atpH-atpI

LSC · 12692-13811 · 1120 bp

Back to Ceratocephala

Pi 0.00179

MarkerSeek score 59.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1120
variable_sites: 2
pi: 0.00179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCAGGTCCAATAGAAGCCA	ATTTTCGCAACTTTAGCCGC	50.0	45.0	58.0	58.0	1252 bp	1.0	79.7
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	ATTTTCGCAACTTTAGCCGC	45.0	45.0	58.0	58.0	1209 bp	1.0	79.6
atpH-atpI_p3	CGCAATACCTTCTACGGCTT	ATTTTCGCAACTTTAGCCGC	50.0	45.0	58.1	58.0	1297 bp	1.0	79.4
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	ATTTTCGCAACTTTAGCCGC	50.0	45.0	57.9	58.0	1265 bp	1.0	79.0
atpH-atpI_p5	AGCCAATCCAGCAGCAATAA	ATTTTCGCAACTTTAGCCGC	45.0	45.0	57.9	58.0	1225 bp	1.0	78.8