Hotspot

rps16-trnQ-UUG

LSC · 5684-6311 · 628 bp

Back to Ceratocephala

Pi 0.00161

MarkerSeek score 60.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 628
variable_sites: 1
pi: 0.00161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCATGCCCTTCAAGCCATAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	704-712 bp	1.0	80.0
rps16-trnQ-UUG_p2	TAGCACAACGGTTGAAACGA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	675-683 bp	1.0	79.9
rps16-trnQ-UUG_p3	TCATGCCCTTCAAGCCATAC	GGTAAGGCATCGGGTTTTGA	50.0	50.0	57.9	58.2	736-744 bp	1.0	79.1
rps16-trnQ-UUG_p4	TAGCACAACGGTTGAAACGA	GGTAAGGCATCGGGTTTTGA	45.0	50.0	58.1	58.2	707-715 bp	1.0	79.1
rps16-trnQ-UUG_p5	TCATGCCCTTCAAGCCATAC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	57.9	57.8	708-716 bp	1.0	78.9