Hotspot

rps2

LSC · 15698-16408 · 711 bp

Back to Cerasus

Pi 0

MarkerSeek score 27.80

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TCTATGGAGTTCCCCCAAAA	GAGAAGAAAAGAAGTGTGGGGA	45.0	45.5	55.7	57.9	777 bp	1.0	44.9
rps2_p2	TTCTATGGAGTTCCCCCAAA	GAGAAGAAAAGAAGTGTGGGGA	45.0	45.5	55.7	57.9	778 bp	1.0	44.9
rps2_p3	TCTATGGAGTTCCCCCAAAA	AGAGAAGAAAAGAAGTGTGGGG	45.0	45.5	55.7	57.9	778 bp	1.0	44.9
rps2_p4	TTCTATGGAGTTCCCCCAAA	AGAGAAGAAAAGAAGTGTGGGG	45.0	45.5	55.7	57.9	779 bp	1.0	44.9
rps2_p5	TCTATGGAGTTCCCCCAAAA	AGAAGAAAAGAAGTGTGGGGA	45.0	42.9	55.7	56.8	776 bp	1.0	43.8