Hotspot

ndhF

SSC · 112400-114646 · 2247 bp

Back to Cerasus

Pi 0.00223

MarkerSeek score 58.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 5
pi: 0.00223
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCCACTTTAAAGAGACACGCT	AACTGACCCCTTTCAACAGA	42.9	45.0	57.3	56.6	2430-2445 bp	1.0	54.9
ndhF_p2	TCCACTTTAAAGAGACACGCT	ACTGACCCCTTTCAACAGAA	42.9	45.0	57.3	56.6	2429-2444 bp	1.0	54.9
ndhF_p3	TCCACTTTAAAGAGACACGCT	AGAACCGGTGAATCAGAAACA	42.9	42.9	57.3	57.5	2401-2416 bp	1.0	54.4
ndhF_p4	TCCACTTTAAAGAGACACGCT	AACTGACCCCTTTCAACAGAA	42.9	42.9	57.3	57.3	2430-2445 bp	1.0	53.0
ndhF_p5	TCCACTTTAAAGAGACACGCT	ACTGACCCCTTTCAACAGAAA	42.9	42.9	57.3	57.3	2429-2444 bp	1.0	53.0