Hotspot

ndhA

SSC · 122740-124970 · 2231 bp

Back to Cerasus

Pi 0.000897

MarkerSeek score 57.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2231
variable_sites: 2
pi: 0.000897
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGACCATAATTCATGAACCCAGT	CCCCGGGTTTTATCAATTTGC	39.1	47.6	57.8	58.1	2458-2460 bp	1.0	45.8
ndhA_p2	ACCCAGTTACCATAGAGAACA	CCCCGGGTTTTATCAATTTGC	42.9	47.6	55.7	58.1	2442-2444 bp	1.0	44.9
ndhA_p3	GAACCCAGTTACCATAGAGAACA	CCCCGGGTTTTATCAATTTGC	43.5	47.6	57.6	58.1	2444-2446 bp	1.0	44.0
ndhA_p4	TGAACCCAGTTACCATAGAGAAC	CCCCGGGTTTTATCAATTTGC	43.5	47.6	57.6	58.1	2445-2447 bp	1.0	44.0
ndhA_p5	AACCCAGTTACCATAGAGAACA	CCCCGGGTTTTATCAATTTGC	40.9	47.6	56.5	58.1	2443-2445 bp	1.0	43.0