Hotspot

trnL-CAA-ndhB

IRb · 96246-96864 · 619 bp

Back to Cerasus

Pi 0.00162

MarkerSeek score 59.70

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 619
variable_sites: 1
pi: 0.00162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	CCAGGAATATCAATGAACCCGA	50.0	45.5	58.0	57.9	705 bp	1.0	63.4
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	CCAGGAATATCAATGAACCCGA	50.0	45.5	58.0	57.9	703 bp	1.0	63.4
trnL-CAA-ndhB_p3	TACCATTTCACCACCAAGGC	CCAGGAATATCAATGAACCCGA	50.0	45.5	58.1	57.9	693 bp	1.0	62.8
trnL-CAA-ndhB_p4	GTCTCGCGTGTCTACCATTT	ACCAGGAATATCAATGAACCCG	50.0	45.5	58.0	58.2	706 bp	1.0	62.6
trnL-CAA-ndhB_p5	CTCGCGTGTCTACCATTTCA	ACCAGGAATATCAATGAACCCG	50.0	45.5	58.0	58.2	704 bp	1.0	62.6