Hotspot

trnF-GAA-ndhJ

LSC · 50010-50794 · 785 bp

Back to Cerasus

Pi 0.00133

MarkerSeek score 63.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 785
variable_sites: 1
pi: 0.00133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	926-959 bp	1.0	82.4
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	890-923 bp	1.0	82.1
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	914-947 bp	1.0	81.3
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	CCACGCCTGAAACGTATCTT	55.0	50.0	58.0	58.3	924-957 bp	1.0	80.6
trnF-GAA-ndhJ_p5	TGAGGATGATGCGATGTGTC	ATCCACGCCTGAAACGTATC	50.0	50.0	57.8	58.1	956-989 bp	1.0	80.4