Hotspot

ycf3-trnS-GGA

LSC · 45900-46521 · 622 bp

Back to Cerasus

Pi 0

MarkerSeek score 60.20

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 622
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGCCTCTTTTTCTCCCGAAG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	806-815 bp	1.0	78.2
ycf3-trnS-GGA_p2	GCCTCTTTTTCTCCCGAAGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	805-814 bp	1.0	78.2
ycf3-trnS-GGA_p3	TGCCTCTTTTTCTCCCGAAG	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.8	57.8	756-765 bp	1.0	77.9
ycf3-trnS-GGA_p4	GCCTCTTTTTCTCCCGAAGT	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.8	57.8	755-764 bp	1.0	77.9
ycf3-trnS-GGA_p5	TGCCTCTTTTTCTCCCGAAG	CGAGTACGGAAAGAGAGGGA	50.0	55.0	57.8	58.3	811-820 bp	1.0	77.4