Hotspot

psbA-trnK-UUU

LSC · 1426-1680 · 255 bp

Back to Cerasus

Pi 0

MarkerSeek score 23.80

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 255
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 23.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TCCAGTTACAAAAGCGACCC	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.1	58.6	358 bp	1.0	74.2
psbA-trnK-UUU_p2	TCCAGTTACAAAAGCGACCC	ACTAGTTCCGGGTTCGAGTC	50.0	55.0	58.1	58.8	348 bp	1.0	72.5
psbA-trnK-UUU_p3	TCCAGTTACAAAAGCGACCC	GACTAGTTCCGGGTTCGAGT	50.0	55.0	58.1	58.8	349 bp	1.0	72.5
psbA-trnK-UUU_p4	ACGGTTTTCAGTGCTGGTTA	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.7	58.6	378 bp	1.0	72.4
psbA-trnK-UUU_p5	TCCAGTTACAAAAGCGACCC	ATCCGACTAGTTCCGGGTTC	50.0	55.0	58.1	58.9	353 bp	1.0	72.0