Hotspot

rpl16

LSC · 133096-134365 · 1270 bp

Back to Cerastium

Pi 0.00945

MarkerSeek score 49.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1270
variable_sites: 30
pi: 0.00945
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACTAAAATCCGCGGTGTCTC	AAACCATTCGCGCGAAAATT	50.0	40.0	58.0	57.9	2050-2099 bp	1.0	83.1
rpl16_p2	CGCACCAAAAACTCGAGTTC	AAACCATTCGCGCGAAAATT	50.0	40.0	58.0	57.9	1867-1916 bp	1.0	83.1
rpl16_p3	GCTATTCGAACCGTCCCTTT	AAACCATTCGCGCGAAAATT	50.0	40.0	58.0	57.9	2135-2184 bp	1.0	82.8
rpl16_p4	ACTAAAATCCGCGGTGTCTC	AAGAACATCTTCGGGTGTCG	50.0	50.0	58.0	57.9	2457-2506 bp	1.0	82.5
rpl16_p5	CGCACCAAAAACTCGAGTTC	AAGAACATCTTCGGGTGTCG	50.0	50.0	58.0	57.9	2274-2323 bp	1.0	82.5