Hotspot

rpl20-rps12

LSC · 121081-121848 · 768 bp

Back to Cerastium

Pi 0.00838

MarkerSeek score 61.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 768
variable_sites: 17
pi: 0.00838
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CGCGAAAGGTTGATGCAAAT	CGCCGAGGAACATGTACTAG	45.0	55.0	58.0	57.9	864-871 bp	1.0	82.2
rpl20-rps12_p2	CGCGAAAGGTTGATGCAAAT	GCCGAGGAACATGTACTAGG	45.0	55.0	58.0	57.5	863-870 bp	1.0	79.1
rpl20-rps12_p3	CGCGAAAGGTTGATGCAAAT	AAATGTCACGAAATCCCCCG	45.0	50.0	58.0	58.5	904-911 bp	1.0	78.7
rpl20-rps12_p4	TGTTCTACGTCTGCGAGCTA	CGCCGAGGAACATGTACTAG	50.0	55.0	58.6	57.9	833-840 bp	1.0	78.0
rpl20-rps12_p5	TTTGTTCTACGTCTGCGAGC	CGCCGAGGAACATGTACTAG	50.0	55.0	58.6	57.9	835-842 bp	1.0	77.8