Hotspot

psaJ-rpl33

LSC · 119317-119746 · 430 bp

Back to Cerastium

Pi 0.0257

MarkerSeek score 59.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 430
variable_sites: 31
pi: 0.0257
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTCCCAGATGCGTTGACA	CGGACATCTTTCCCCTTAGC	45.0	55.0	58.0	58.0	487-496 bp	1.0	87.4
psaJ-rpl33_p2	TGAATGCTCCGTTTCGTAGG	CGGACATCTTTCCCCTTAGC	50.0	55.0	58.0	58.0	763-801 bp	1.0	87.3
psaJ-rpl33_p3	TGAATGCTCCGTTTCGTAGG	CCCTTCGTTGAACGGATGAT	50.0	50.0	58.0	58.0	968-1006 bp	1.0	87.1
psaJ-rpl33_p4	TTTTCCCAGATGCGTTGACA	CCCTTCGTTGAACGGATGAT	45.0	50.0	58.0	58.0	692-701 bp	1.0	85.7
psaJ-rpl33_p5	TTGGGTCTTTAGCCGGTCTA	CCCTTCGTTGAACGGATGAT	50.0	50.0	58.1	58.0	731-740 bp	1.0	85.1