Hotspot

psbE-petL

LSC · 116804-117975 · 1172 bp

Back to Cerastium

Pi 0.0103

MarkerSeek score 59.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1172
variable_sites: 32
pi: 0.0103
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	CCGAAATAACTTGTTAGAGTAGGC	50.0	41.7	57.8	57.4	1208-1241 bp	1.0	47.0
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	CCGAAATAACTTGTTAGAGTAGGC	55.0	41.7	58.2	57.4	1203-1236 bp	1.0	46.6
psbE-petL_p3	CGCAAAAGAACGTTCTCCTG	CCGAAATAACTTGTTAGAGTAGGC	50.0	41.7	57.7	57.4	1214-1247 bp	1.0	45.8
psbE-petL_p4	TCTCCTGTGCTTCCAGACAT	CCGAAATAACTTGTTAGAGTAGGC	50.0	41.7	58.4	57.4	1201-1234 bp	1.0	45.4
psbE-petL_p5	TTCTCCTGTGCTTCCAGACA	CCGAAATAACTTGTTAGAGTAGGC	50.0	41.7	58.6	57.4	1202-1235 bp	1.0	44.9