Hotspot

petA-psbJ

LSC · 115108-116007 · 900 bp

Back to Cerastium

Pi 0.0131

MarkerSeek score 63.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 900
variable_sites: 33
pi: 0.0131
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGCGTACAAGGTCTTTTGT	ATTCTGGATTGGGCTCATCG	45.0	50.0	58.1	57.7	1013-1038 bp	1.0	83.9
petA-psbJ_p2	CCATTACGCGTACAAGGTCT	ATTCTGGATTGGGCTCATCG	50.0	50.0	57.7	57.7	1018-1043 bp	1.0	82.0
petA-psbJ_p3	CCCATTACGCGTACAAGGTC	ATTCTGGATTGGGCTCATCG	55.0	50.0	58.7	57.7	1019-1044 bp	1.0	78.5
petA-psbJ_p4	GACCCATTACGCGTACAAGG	ATTCTGGATTGGGCTCATCG	55.0	50.0	58.7	57.7	1021-1046 bp	1.0	78.5
petA-psbJ_p5	ACGCGTACAAGGTCTTTTGT	TGGATTGGGCTCATCGCTAT	45.0	50.0	58.1	58.9	1009-1034 bp	1.0	78.5