Hotspot

ndhC-trnV-UAC

LSC · 103063-104198 · 1136 bp

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Pi 0.0142

MarkerSeek score 62.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1136
variable_sites: 43
pi: 0.0142
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGCCCAGAAAATGTCATATTCA	GTTCGAGTCCGTATAGCCCT	36.4	55.0	56.0	58.7	1166-1208 bp	1.0	49.7
ndhC-trnV-UAC_p2	AAAATGCCCAGAAAATGTCA	GTTCGAGTCCGTATAGCCCT	35.0	55.0	53.9	58.7	1170-1212 bp	1.0	48.7
ndhC-trnV-UAC_p3	AAAAATGCCCAGAAAATGTCA	GTTCGAGTCCGTATAGCCCT	33.3	55.0	54.8	58.7	1171-1213 bp	1.0	47.7
ndhC-trnV-UAC_p4	TGCCCAGAAAATGTCATATTCA	TTCGAGTCCGTATAGCCCTA	36.4	50.0	56.0	57.1	1165-1207 bp	1.0	47.7
ndhC-trnV-UAC_p5	AAATGCCCAGAAAATGTCAT	GTTCGAGTCCGTATAGCCCT	35.0	55.0	53.7	58.7	1169-1211 bp	1.0	46.9