Hotspot

trnL-UAA-trnF-GAA

LSC · 100434-100808 · 375 bp

Back to Cerastium

Pi 0.0162

MarkerSeek score 56.20

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 375
variable_sites: 18
pi: 0.0162
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	461-477 bp	1.0	81.8
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	476-492 bp	1.0	81.3
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	AATCATGTGCCAGGAACCAG	50.0	50.0	57.5	58.2	482-498 bp	1.0	80.7
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	CATGTGCCAGGAACCAGATT	50.0	50.0	57.5	58.2	479-495 bp	1.0	80.7
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	AAATCATGTGCCAGGAACCA	50.0	45.0	57.5	57.7	483-499 bp	1.0	79.5