Hotspot

trnD-GUC-trnY-GUA

LSC · 83802-84179 · 378 bp

Back to Cerastium

Pi 0.0198

MarkerSeek score 61.30

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 378
variable_sites: 22
pi: 0.0198
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GCGGTGCTCTAACCAATTGA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.6	57.8	440-455 bp	1.0	81.2
trnD-GUC-trnY-GUA_p2	GCGGTGCTCTAACCAATTGA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.6	57.8	439-454 bp	1.0	81.2
trnD-GUC-trnY-GUA_p3	GCGGTGCTCTAACCAATTGA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.6	58.4	461-476 bp	1.0	79.9
trnD-GUC-trnY-GUA_p4	GCGGTGCTCTAACCAATTGA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.6	57.6	460-475 bp	1.0	79.8
trnD-GUC-trnY-GUA_p5	GCGGTGCTCTAACCAATTGA	CGGACTGTAAATTCGTTGGC	50.0	50.0	58.6	57.5	456-471 bp	1.0	78.7