Hotspot

psbM-trnD-GUC

LSC · 83170-83727 · 558 bp

Back to Cerastium

Pi 0.0226

MarkerSeek score 57.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 558
variable_sites: 30
pi: 0.0226
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAAGAGCGCAGTAGC	AGATTTTTCCGGGGTCGATG	50.0	50.0	58.1	58.0	1142-1171 bp	1.0	85.4
psbM-trnD-GUC_p2	AATGAAGAGCGCAGTAGCAA	AGATTTTTCCGGGGTCGATG	45.0	50.0	57.9	58.0	1140-1169 bp	1.0	85.2
psbM-trnD-GUC_p3	AGAATGAAGAGCGCAGTAGC	CCTTCCCAAGCAGACGATAC	50.0	55.0	58.1	58.1	1010-1022 bp	0.833333	75.6
psbM-trnD-GUC_p4	AATGAAGAGCGCAGTAGCAA	CCTTCCCAAGCAGACGATAC	45.0	55.0	57.9	58.1	1008-1020 bp	0.833333	75.3
psbM-trnD-GUC_p5	AGAATGAAGAGCGCAGTAGC	TCCTTCCCAAGCAGACGATA	50.0	50.0	58.1	58.1	1011-1023 bp	0.833333	74.9