Hotspot

trnR-UCU-atpA

LSC · 65079-65197 · 119 bp

Back to Cerastium

Pi 0.0303
MarkerSeek score 60.40
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
119
variable_sites
8
pi
0.0303
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGTTCAAATCCTATTGGACGC TGGAACGTTTTCTACTTCAGGA 47.6 40.9 57.3 57.3 156-183 bp 1.0 52.8
trnR-UCU-atpA_p2 GGTTCAAATCCTATTGGACGC TGGAACGTTTTCTACTTCAGGAA 47.6 39.1 57.3 58.0 156-183 bp 1.0 50.0
trnR-UCU-atpA_p3 TGGATAGGACAGAGGTCTTCT TGGAACGTTTTCTACTTCAGGA 47.6 40.9 56.9 57.3 191-218 bp 1.0 49.3
trnR-UCU-atpA_p4 GGTTCAAATCCTATTGGACGC TCAAGAACAGATGGAACGTT 47.6 40.0 57.3 54.9 167-194 bp 1.0 49.3
trnR-UCU-atpA_p5 GGTTCAAATCCTATTGGACGC TTCAAGAACAGATGGAACGT 47.6 40.0 57.3 54.9 168-195 bp 1.0 49.3