Hotspot

trnS-GCU-trnG-UCC

LSC · 63329-64104 · 776 bp

Back to Cerastium

Pi 0.018

MarkerSeek score 67.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 776
variable_sites: 38
pi: 0.018
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CACGGAAAGAGAGGGATTCG	ACGAATCACACTTTTACCACT	55.0	38.1	58.1	55.5	871-900 bp	1.0	58.6
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	870-899 bp	1.0	57.9
trnS-GCU-trnG-UCC_p3	CACGGAAAGAGAGGGATTCG	AGAACGAATCACACTTTTACCAC	55.0	39.1	58.1	57.3	874-903 bp	1.0	57.5
trnS-GCU-trnG-UCC_p4	CACGGAAAGAGAGGGATTCG	GAACGAATCACACTTTTACCACT	55.0	39.1	58.1	57.3	873-902 bp	1.0	57.5
trnS-GCU-trnG-UCC_p5	CACGGAAAGAGAGGGATTCG	AGGGTGTAATAGAACGAATCACA	55.0	39.1	58.1	57.3	884-913 bp	1.0	57.4