Hotspot

ycf4

Genome · 8222-8767 · 546 bp

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Pi 0.00387

MarkerSeek score 50.30

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: Genome
length_bp: 546
variable_sites: 6
pi: 0.00387
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	ATGAGAGTGACTTTGGGAGT	CGTTTGGGTGTTGGAGAAGA	45.0	50.0	55.8	58.0	648-649 bp	1.0	62.7
ycf4_p2	ATGAGAGTGACTTTGGGAGT	TCGTTTGGGTGTTGGAGAAG	45.0	50.0	55.8	58.0	649-650 bp	1.0	62.7
ycf4_p3	ATGAGAGTGACTTTGGGAGT	TTCGTTTGGGTGTTGGAGAA	45.0	45.0	55.8	57.6	650-651 bp	1.0	59.6
ycf4_p4	ATGAGAGTGACTTTGGGAGT	ATTCGTTTGGGTGTTGGAGA	45.0	45.0	55.8	57.4	651-652 bp	1.0	57.8
ycf4_p5	GTGTTTGGTCACTCTTTCAACT	CGTTTGGGTGTTGGAGAAGA	40.9	50.0	57.0	58.0	617-618 bp	1.0	56.9