Hotspot

ycf2

Genome · 121116-128309 · 7194 bp

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Pi 0.00275

MarkerSeek score 38.50

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 7194
variable_sites: 52
pi: 0.00275
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TCCATCCGGGATCTGATGAA	TTGGGTTTTCTCAAGGTCCG	50.0	50.0	57.9	58.0	899-1088 bp	1.0	84.8
ycf2_p2	TCCATCCGGGATCTGATGAA	TCTCAGATAACCAAGGCCCA	50.0	50.0	57.9	58.0	849-1038 bp	1.0	84.8
ycf2_p3	TCCATCCGGGATCTGATGAA	TATCAACGTCGCTTGCTCAA	50.0	45.0	57.9	57.9	934-1123 bp	1.0	84.3
ycf2_p4	TCCATCCGGGATCTGATGAA	AAGTTGGGGAAGTCATTCGG	50.0	50.0	57.9	57.8	827-1016 bp	1.0	83.5
ycf2_p5	TCCATCCGGGATCTGATGAA	GGTTTTCTCAAGGTCCGACA	50.0	50.0	57.9	57.8	896-1085 bp	1.0	83.0