Hotspot

ycf1

Genome · 82514-90805 · 8292 bp

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Pi 0.00808

MarkerSeek score 45.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 8292
variable_sites: 172
pi: 0.00808
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCAAGGGACCGAAAATAGC	TTCTTGGAAAACCAGGGAGC	50.0	50.0	57.9	58.0	748-1294 bp	1.0	90.5
ycf1_p2	ACCAAGGGACCGAAAATAGC	TCCCTTTGCCAATATCGGTG	50.0	50.0	57.9	57.9	833-1379 bp	1.0	89.6
ycf1_p3	ACCAAGGGACCGAAAATAGC	GTCAATGTCTCCCTTTGCCA	50.0	50.0	57.9	58.1	842-1388 bp	1.0	89.2
ycf1_p4	ACCAAGGGACCGAAAATAGC	CGGTCATTAAAGTACCCCGG	50.0	55.0	57.9	58.1	997-1543 bp	1.0	88.8
ycf1_p5	ACCAAGGGACCGAAAATAGC	ACAGTTTGATTCGATCCGCA	50.0	45.0	57.9	57.9	963-1509 bp	1.0	88.6