Hotspot

trnI-GAU-rrn16

Genome · 111811-113217 · 1407 bp

Back to Cephalotaxus

Pi 0.0272

MarkerSeek score 66.00

Primer available yes

Label trnI-GAU-rrn16

Feature Metadata

label_name: trnI-GAU-rrn16
feature_type: igs
region: Genome
length_bp: 1407
variable_sites: 90
pi: 0.0272
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-rrn16_p1	ACAACGACGCGATTATCAGG	GTAGCTGTACTGGAAGGTGC	50.0	55.0	58.4	58.0	1248-1611 bp	1.0	85.4
trnI-GAU-rrn16_p2	ACAACGACGCGATTATCAGG	GACCGGAGTGAAGTCGTAAC	50.0	55.0	58.4	58.0	1271-1634 bp	1.0	85.2
trnI-GAU-rrn16_p3	CACAACGACGCGATTATCAG	GTAGCTGTACTGGAAGGTGC	50.0	55.0	57.4	58.0	1249-1612 bp	1.0	84.1
trnI-GAU-rrn16_p4	CACAACGACGCGATTATCAG	GACCGGAGTGAAGTCGTAAC	50.0	55.0	57.4	58.0	1272-1635 bp	1.0	83.9
trnI-GAU-rrn16_p5	ACAACGACGCGATTATCAGG	TGACCGGAGTGAAGTCGTAA	50.0	50.0	58.4	58.4	1272-1635 bp	1.0	82.3