Hotspot

trnH-GUG-psbA

Genome · 77005-77291 · 287 bp

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Pi 0.0209

MarkerSeek score 46.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: Genome
length_bp: 287
variable_sites: 12
pi: 0.0209
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TCTGGTCGGTTTTTCATCGG	GTAACGCTCACAACTTCCCT	50.0	50.0	58.2	57.8	443 bp	1.0	79.3
trnH-GUG-psbA_p2	TCTGGTCGGTTTTTCATCGG	TGGAAGTTATGCACGAACGT	50.0	45.0	58.2	57.8	461 bp	1.0	79.2
trnH-GUG-psbA_p3	TCTGGTCGGTTTTTCATCGG	GTTATGCACGAACGTAACGC	50.0	50.0	58.2	58.2	456 bp	1.0	78.8
trnH-GUG-psbA_p4	TCTGGTCGGTTTTTCATCGG	TTATGCACGAACGTAACGCT	50.0	45.0	58.2	58.3	455 bp	1.0	78.1
trnH-GUG-psbA_p5	ACAATCCACTGCCTTTGGAC	TGGAAGTTATGCACGAACGT	50.0	45.0	58.4	57.8	404 bp	1.0	78.1