Hotspot

psaA-ycf3

Genome · 59602-60199 · 598 bp

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Pi 0.00474

MarkerSeek score 50.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: Genome
length_bp: 598
variable_sites: 10
pi: 0.00474
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	ATAGGATCCCTCTCCACCAC	TGGTTTGATCAAGCTGCTGA	55.0	45.0	57.6	57.7	753-767 bp	1.0	76.7
psaA-ycf3_p2	ATAGGATCCCTCTCCACCAC	CGGAGGCTTGGTTTGATCAA	55.0	50.0	57.6	58.5	761-775 bp	1.0	75.2
psaA-ycf3_p3	ATAGGATCCCTCTCCACCAC	TGGAAACAAGCTATAGCGCT	55.0	45.0	57.6	57.3	729-743 bp	1.0	73.5
psaA-ycf3_p4	AAATGACCAGGTTTAGCCCA	TGGTTTGATCAAGCTGCTGA	45.0	45.0	57.0	57.7	792-806 bp	1.0	72.0
psaA-ycf3_p5	TGACCAGGTTTAGCCCATTT	TGGTTTGATCAAGCTGCTGA	45.0	45.0	57.0	57.7	789-803 bp	1.0	72.0