Hotspot

psbK-psbI

Genome · 27047-27669 · 623 bp

Back to Cephalotaxus

Pi 0.0051

MarkerSeek score 47.40

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: Genome
length_bp: 623
variable_sites: 9
pi: 0.0051
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCTGCCGTAAGTTTTCG	TCACTGTTCTTTACGCCCAG	50.0	50.0	58.0	57.8	761-780 bp	1.0	79.8
psbK-psbI_p2	AGCTGCCGTAAGTTTTCGAT	TCACTGTTCTTTACGCCCAG	45.0	50.0	57.9	57.8	759-778 bp	1.0	79.5
psbK-psbI_p3	AAGCTGCCGTAAGTTTTCGA	TCACTGTTCTTTACGCCCAG	45.0	50.0	58.1	57.8	760-779 bp	1.0	79.2
psbK-psbI_p4	GAGGCTTATGCGATTTCCGA	TCACTGTTCTTTACGCCCAG	50.0	50.0	58.1	57.8	842-861 bp	1.0	79.0
psbK-psbI_p5	CAAGCTGCCGTAAGTTTTCG	CTGTTCTTTACGCCCAGGAT	50.0	50.0	58.0	57.6	758-777 bp	1.0	77.9