Hotspot

chlB-trnQ-UUG

Genome · 26273-26455 · 183 bp

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Pi 0.014

MarkerSeek score 46.90

Primer available yes

Label chlB-trnQ-UUG

Feature Metadata

label_name: chlB-trnQ-UUG
feature_type: igs
region: Genome
length_bp: 183
variable_sites: 7
pi: 0.014
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB-trnQ-UUG_p1	GAGCAGGACCAGCATACATC	TGGTCCTGTTATTGCGAAGG	55.0	50.0	58.1	57.9	260-261 bp	1.0	80.3
chlB-trnQ-UUG_p2	GAGCAGGACCAGCATACATC	GGTCCTGTTATTGCGAAGGT	55.0	50.0	58.1	57.9	259-260 bp	1.0	80.3
chlB-trnQ-UUG_p3	AGAGGAGCATGCATTATGGC	TGGTCCTGTTATTGCGAAGG	50.0	50.0	58.1	57.9	324-325 bp	1.0	80.1
chlB-trnQ-UUG_p4	AGAGGAGCATGCATTATGGC	GGTCCTGTTATTGCGAAGGT	50.0	50.0	58.1	57.9	323-324 bp	1.0	80.1
chlB-trnQ-UUG_p5	AGCTACTCGTAGGGTTCCAA	TGGTCCTGTTATTGCGAAGG	50.0	50.0	57.8	57.9	283-284 bp	1.0	79.3