Hotspot

clpP-accD

Genome · 18854-19778 · 925 bp

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Pi 0.0109
MarkerSeek score 44.90
Primer available yes
Label clpP-accD

Feature Metadata

label_name
clpP-accD
feature_type
igs
region
Genome
length_bp
925
variable_sites
28
pi
0.0109
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-accD_p1 ATTATAGCGGATACTCGTAAG CATGGTTCCACGGGCTATTT 38.1 50.0 52.2 58.2 1022-1130 bp 1.0 42.3
clpP-accD_p2 ATTATAGCGGATACTCGTAAG TTGATACCATGGTTCCACGG 38.1 50.0 52.2 57.6 1029-1137 bp 1.0 42.3
clpP-accD_p3 ATTATAGCGGATACTCGTAAG ATGGTTCCACGGGCTATTTT 38.1 45.0 52.2 57.5 1021-1129 bp 1.0 42.3
clpP-accD_p4 TATTATAGCGGATACTCGTAAG CATGGTTCCACGGGCTATTT 36.4 50.0 52.3 58.2 1023-1131 bp 1.0 42.3
clpP-accD_p5 ATTATAGCGGATACTCGTAAG TGGTTCCACGGGCTATTTTA 38.1 45.0 52.2 56.8 1020-1128 bp 1.0 42.3