Hotspot

petG-trnW-CCA

Genome · 14495-14613 · 119 bp

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Pi 0.0434

MarkerSeek score 59.00

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: Genome
length_bp: 119
variable_sites: 10
pi: 0.0434
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	CGACGTGGTGATCAATTGGA	CTCCAAAACCTGATGCCGTA	50.0	50.0	58.3	57.9	188 bp	1.0	81.2
petG-trnW-CCA_p2	CGACGTGGTGATCAATTGGA	TCCAAAACCTGATGCCGTAG	50.0	50.0	58.3	57.9	187 bp	1.0	81.2
petG-trnW-CCA_p3	CGACGTGGTGATCAATTGGA	AGCGCTCTTAGTTCAGTTGG	50.0	50.0	58.3	57.9	221 bp	1.0	80.7
petG-trnW-CCA_p4	CGACGTGGTGATCAATTGGA	GTTCAGTTGGGTAGAACGCA	50.0	50.0	58.3	58.1	211 bp	1.0	80.5
petG-trnW-CCA_p5	CGACGTGGTGATCAATTGGA	TTCAGTTGGGTAGAACGCAG	50.0	50.0	58.3	57.8	210 bp	1.0	80.3