Hotspot

petA-psbJ

Genome · 11401-12163 · 763 bp

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Pi 0.00371

MarkerSeek score 47.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 763
variable_sites: 10
pi: 0.00371
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCATCTGTCATTTTGGCACA	TCCGGATTAGGATCATCCCT	45.0	50.0	57.3	57.0	871 bp	1.0	66.9
petA-psbJ_p2	AGTGCAATTGGTCGAGATGA	TCCGGATTAGGATCATCCCT	45.0	50.0	57.2	57.0	815 bp	1.0	66.7
petA-psbJ_p3	GCATCTGTCATTTTGGCACA	CGGAAGGATCCCTCTTTGGT	45.0	55.0	57.3	59.1	956 bp	1.0	66.3
petA-psbJ_p4	AGTGCAATTGGTCGAGATGA	CGGAAGGATCCCTCTTTGGT	45.0	55.0	57.2	59.1	900 bp	1.0	66.0
petA-psbJ_p5	GCATCTGTCATTTTGGCACA	GGAAGGATCCCTCTTTGGTT	45.0	50.0	57.3	56.8	955 bp	1.0	65.4