Hotspot

rpoC2

LSC · 16833-20975 · 4143 bp

Back to Cephalantheropsis

Pi 0.000724

MarkerSeek score 54.30

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4143
variable_sites: 3
pi: 0.000724
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TGGACATGATTCGATAAGGT	TGATGGTTATCATGCGACCG	40.0	50.0	53.8	58.1	284 bp	1.0	45.9
rpoC2_p2	TGGACATGATTCGATAAGGT	GATGGTTATCATGCGACCGA	40.0	50.0	53.8	57.9	283 bp	1.0	45.8
rpoC2_p3	TGGACATGATTCGATAAGGTT	TGATGGTTATCATGCGACCG	38.1	50.0	54.7	58.1	284 bp	1.0	44.9
rpoC2_p4	TTGGACATGATTCGATAAGGT	TGATGGTTATCATGCGACCG	38.1	50.0	54.7	58.1	285 bp	1.0	44.9
rpoC2_p5	TGGACATGATTCGATAAGGTT	GATGGTTATCATGCGACCGA	38.1	50.0	54.7	57.9	283 bp	1.0	44.8