Hotspot

psbA

LSC · 166-1227 · 1062 bp

Back to Cephalantheropsis

Pi 0.000942

MarkerSeek score 59.30

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AGCAATCCCCCAAGATCTTG	GTGCTTGGGAGTCCTTGAAA	50.0	50.0	57.6	58.0	1187 bp	1.0	76.3
psbA_p2	CAAGATCTTGGGGGATTGCT	GTGCTTGGGAGTCCTTGAAA	50.0	50.0	57.6	58.0	1177 bp	1.0	76.3
psbA_p3	AGCAATCCCCCAAGATCTTG	AATACGTGTGCTTGGGAGTC	50.0	50.0	57.6	57.9	1194 bp	1.0	75.7
psbA_p4	CAAGATCTTGGGGGATTGCT	AATACGTGTGCTTGGGAGTC	50.0	50.0	57.6	57.9	1184 bp	1.0	75.7
psbA_p5	CAACAAGATAGCAATCCCCCA	GTGCTTGGGAGTCCTTGAAA	47.6	50.0	58.0	58.0	1196 bp	1.0	71.8