Hotspot

ndhK

LSC · 49959-50732 · 774 bp

Back to Cephalantheropsis

Pi 0.00129

MarkerSeek score 59.50

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 774
variable_sites: 1
pi: 0.00129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	TGCATCTTCTTCATTTCTCCCA	TGGGCAATGAGCTTTGATGT	40.9	45.0	57.7	58.1	970 bp	1.0	61.1
ndhK_p2	TGCATCTTCTTCATTTCTCCCA	ATCCATGGGCAATGAGCTTT	40.9	45.0	57.7	57.8	975 bp	1.0	60.3
ndhK_p3	TGCATCTTCTTCATTTCTCCCA	GGTTCAGTTTATGCATGGCG	40.9	50.0	57.7	57.8	895 bp	1.0	60.1
ndhK_p4	GCATCTTCTTCATTTCTCCCAC	TGGGCAATGAGCTTTGATGT	45.5	45.0	57.4	58.1	969 bp	1.0	58.5
ndhK_p5	GCATCTTCTTCATTTCTCCCAC	ATCCATGGGCAATGAGCTTT	45.5	45.0	57.4	57.8	974 bp	1.0	57.7