Hotspot

clpP-psbB

LSC · 73793-74467 · 675 bp

Back to Cephalantheropsis

Pi 0
MarkerSeek score 60.20
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
675
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 CGCACTATACGTCAACCCAA GGCCCAACCAGAAACTAGAG 50.0 55.0 58.0 57.9 843-853 bp 1.0 80.0
clpP-psbB_p2 TATACGTCAACCCAAGCTGC GGCCCAACCAGAAACTAGAG 50.0 55.0 58.0 57.9 838-848 bp 1.0 79.9
clpP-psbB_p3 CGCACTATACGTCAACCCAA CGATACCAAGGCAAACCCAT 50.0 50.0 58.0 58.2 761-771 bp 1.0 79.1
clpP-psbB_p4 TATACGTCAACCCAAGCTGC CGATACCAAGGCAAACCCAT 50.0 50.0 58.0 58.2 756-766 bp 1.0 79.0
clpP-psbB_p5 CTTTTGGAACACCAATGGGC GGCCCAACCAGAAACTAGAG 50.0 55.0 57.8 57.9 789-799 bp 1.0 78.7