Hotspot

atpB-rbcL

LSC · 55152-56103 · 952 bp

Back to Cephalantheropsis

Pi 0.00105

MarkerSeek score 59.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 952
variable_sites: 1
pi: 0.00105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGCCCGGTGGAAAAATAACA	GGTGACATAAGTCCCTCCCT	45.0	55.0	58.0	58.1	1096-1101 bp	1.0	79.1
atpB-rbcL_p2	ATCTTGCCCGGTGGAAAAAT	GGTGACATAAGTCCCTCCCT	45.0	55.0	58.1	58.1	1100-1105 bp	1.0	78.6
atpB-rbcL_p3	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	1068-1073 bp	1.0	78.0
atpB-rbcL_p4	TGCCCGGTGGAAAAATAACA	TGGTGACATAAGTCCCTCCC	45.0	55.0	58.0	58.4	1097-1102 bp	1.0	76.7
atpB-rbcL_p5	CGATACGCCCTAGGTTTTGT	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.7	58.1	1051-1056 bp	1.0	76.7