Hotspot

ndhC-trnV-UAC

LSC · 51086-52165 · 1080 bp

Back to Cephalantheropsis

Pi 0.000927

MarkerSeek score 59.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1080
variable_sites: 1
pi: 0.000927
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CGGGGCTAAAATCCCAGAAA	CCGAGAATGTCTACGGTTCG	50.0	55.0	57.9	58.2	1214-1215 bp	1.0	77.6
ndhC-trnV-UAC_p2	TCTGGACCTTCCCTAATCGG	CCGAGAATGTCTACGGTTCG	55.0	55.0	58.2	58.2	1231-1232 bp	1.0	77.0
ndhC-trnV-UAC_p3	AATCGGGGCTAAAATCCCAG	CCGAGAATGTCTACGGTTCG	50.0	55.0	57.6	58.2	1217-1218 bp	1.0	75.8
ndhC-trnV-UAC_p4	CGGGGCTAAAATCCCAGAAA	AGCCCAATGCAACAAAATGC	50.0	45.0	57.9	58.5	1293-1294 bp	1.0	75.2
ndhC-trnV-UAC_p5	TCTGGACCTTCCCTAATCGG	AGCCCAATGCAACAAAATGC	55.0	45.0	58.2	58.5	1310-1311 bp	1.0	74.6