Hotspot

rps8

LSC · 84730-85125 · 396 bp

Back to Cephalanthera

Pi 0.00303

MarkerSeek score 29.80

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 396
variable_sites: 3
pi: 0.00303
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	ATAAAACTGCGCCGGATTCT	AATCAAAGAAGCAGTGCCCA	45.0	45.0	58.0	58.0	1162-1201 bp	1.0	82.2
rps8_p2	ATAAAACTGCGCCGGATTCT	ATCAAAGAAGCAGTGCCCAA	45.0	45.0	58.0	58.0	1161-1200 bp	1.0	82.2
rps8_p3	ATAAAACTGCGCCGGATTCT	CGATTGCTCGGGAATTGAGA	45.0	50.0	58.0	58.1	986-1025 bp	1.0	82.2
rps8_p4	ATAAAACTGCGCCGGATTCT	TTGCTCGGGAATTGAGACAG	45.0	50.0	58.0	57.9	983-1022 bp	1.0	81.8
rps8_p5	ATAAAACTGCGCCGGATTCT	GCTCGGGAATTGAGACAGTT	45.0	50.0	58.0	57.9	981-1020 bp	1.0	81.8