Hotspot

trnV-GAC-rps12

IRa · 144597-146343 · 1747 bp

Back to Cephalanthera

Pi 0.00179

MarkerSeek score 61.00

Primer available yes

Label trnV-GAC-rps12

Feature Metadata

label_name: trnV-GAC-rps12
feature_type: igs
region: IRa
length_bp: 1747
variable_sites: 8
pi: 0.00179
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rps12_p1	GATGACTTCCACCACGTCAA	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.8	57.7	1839-2014 bp	1.0	77.9
trnV-GAC-rps12_p2	AACTGATGACTTCCACCACG	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.8	57.7	1843-2018 bp	1.0	77.8
trnV-GAC-rps12_p3	ATGACTTCCACCACGTCAAG	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.8	57.7	1838-2013 bp	1.0	77.8
trnV-GAC-rps12_p4	CTGATGACTTCCACCACGTC	TGGCAACTTTACGTAAGGCA	55.0	45.0	58.3	57.7	1841-2016 bp	1.0	76.9
trnV-GAC-rps12_p5	GTCAAGGTGACACTCTACCG	TGGCAACTTTACGTAAGGCA	55.0	45.0	57.7	57.7	1824-1999 bp	1.0	76.4