Hotspot

rps15-ndhH

SSC · 120846-120988 · 143 bp

Back to Cephalanthera

Pi 0.0177

MarkerSeek score 62.10

Primer available yes

Label rps15-ndhH

Feature Metadata

label_name: rps15-ndhH
feature_type: igs
region: SSC
length_bp: 143
variable_sites: 7
pi: 0.0177
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhH_p1	GGGAACCGAAATTCGTTAAT	TCTTGTAGCCGGTACAGTCA	40.0	50.0	54.0	58.1	193 bp	0.166667	13.9
rps15-ndhH_p2	GGGAACCGAAATTCGTTAAT	ATGCATTGATGGGTGGTGAG	40.0	50.0	54.0	58.2	241 bp	0.166667	12.7
rps15-ndhH_p3	GGGAACCGAAATTCGTTAATT	TCTTGTAGCCGGTACAGTCA	38.1	50.0	54.8	58.1	193 bp	0.166667	12.7
rps15-ndhH_p4	GGGAACCGAAATTCGTTAAT	CATTGATGGGTGGTGAGGAC	40.0	55.0	54.0	58.2	238 bp	0.166667	12.6
rps15-ndhH_p5	GGGAACCGAAATTCGTTAAT	GGTGGTGAGGACCCATATTG	40.0	55.0	54.0	57.7	230 bp	0.166667	11.8