Hotspot

rpl20-rps12

LSC · 72656-73444 · 789 bp

Back to Cephalanthera

Pi 0.00561

MarkerSeek score 64.20

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 789
variable_sites: 12
pi: 0.00561
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	AGTCTTGAATGAGCCCCTCT	GAAATCTCCCGCTCTTCGAG	50.0	55.0	58.0	58.2	894-939 bp	1.0	83.5
rpl20-rps12_p2	TGAGCCCCTCTAAAGGTTGA	GAAATCTCCCGCTCTTCGAG	50.0	55.0	58.0	58.2	885-930 bp	1.0	83.5
rpl20-rps12_p3	AGTCTTGAATGAGCCCCTCT	TAGAAACGCAAGACAGCCAA	50.0	45.0	58.0	57.8	927-972 bp	1.0	82.6
rpl20-rps12_p4	TGAGCCCCTCTAAAGGTTGA	TAGAAACGCAAGACAGCCAA	50.0	45.0	58.0	57.8	918-963 bp	1.0	82.6
rpl20-rps12_p5	AGTCTTGAATGAGCCCCTCT	TTAGAAACGCAAGACAGCCA	50.0	45.0	58.0	57.8	928-973 bp	1.0	82.5