Hotspot

trnE-UUC-trnT-GGU

LSC · 31769-33504 · 1736 bp

Back to Cephalanthera

Pi 0.0126
MarkerSeek score 64.50
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1736
variable_sites
56
pi
0.0126
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 1764-1848 bp 1.0 85.1
trnE-UUC-trnT-GGU_p2 CGTTGCCTCCTTGAAAGAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.8 58.1 1743-1827 bp 1.0 84.6
trnE-UUC-trnT-GGU_p3 CGTTGCCTCCTTGAAAGAGA TGGCGTTACTCTACCACTGA 50.0 50.0 57.8 58.1 1740-1824 bp 1.0 84.4
trnE-UUC-trnT-GGU_p4 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 1742-1826 bp 1.0 84.2
trnE-UUC-trnT-GGU_p5 CGTTGCCTCCTTGAAAGAGA CCGATGACTTACGCCTTACC 50.0 55.0 57.8 58.2 1761-1845 bp 1.0 83.9