Hotspot

psbM-trnD-GUC

LSC · 30170-31266 · 1097 bp

Back to Cephalanthera

Pi 0.0062

MarkerSeek score 64.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1097
variable_sites: 18
pi: 0.0062
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CAGCGCAGTAGCAATAAACG	CCATGCATATGGCTGGGATT	50.0	50.0	57.9	58.1	1077-1235 bp	1.0	84.0
psbM-trnD-GUC_p2	CAGCGCAGTAGCAATAAACG	AGTTCAATCGGTCAGAGCAC	50.0	50.0	57.9	57.9	1055-1213 bp	1.0	83.9
psbM-trnD-GUC_p3	TTGGCTGACTGTTTTGACGT	CCATGCATATGGCTGGGATT	45.0	50.0	58.3	58.1	1134-1292 bp	1.0	82.6
psbM-trnD-GUC_p4	ATGAACAGCGCAGTAGCAAT	CCATGCATATGGCTGGGATT	45.0	50.0	58.3	58.1	1082-1240 bp	1.0	82.6
psbM-trnD-GUC_p5	TTGGCTGACTGTTTTGACGT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1112-1270 bp	1.0	82.4