Hotspot

trnC-GCA-petN

LSC · 28215-29222 · 1008 bp

Back to Cephalanthera

Pi 0.0147
MarkerSeek score 61.60
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
1008
variable_sites
40
pi
0.0147
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 ATTTAATTTGGCGGCATGGC GCTCTAAAGCCCACTCCTTC 45.0 55.0 58.0 58.0 1144-1195 bp 1.0 86.2
trnC-GCA-petN_p2 TCTTTCGGAAGTACATGCCG GCTCTAAAGCCCACTCCTTC 50.0 55.0 58.0 58.0 1783-1872 bp 1.0 85.2
trnC-GCA-petN_p3 AAGTTTTTAAGCGACGCGTG GCTCTAAAGCCCACTCCTTC 45.0 55.0 58.0 58.0 1382-1450 bp 0.833333 78.4
trnC-GCA-petN_p4 TCTTTCGGAAGTACATGCCG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.0 58.2 1717-1806 bp 0.833333 77.6
trnC-GCA-petN_p5 GCGCAACATAGGTTAGGTCA GCTCTAAAGCCCACTCCTTC 50.0 55.0 58.0 58.0 1722-1800 bp 0.833333 76.7