Hotspot

trnS-GCU-trnG-GCC

LSC · 8670-9426 · 757 bp

Back to Cephalanthera

Pi 0.0096

MarkerSeek score 61.60

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 757
variable_sites: 18
pi: 0.0096
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	TTAGTCCCCTCAGCCATCTC	ACGAATCACACTTTTACCACT	55.0	38.1	58.2	55.5	798-822 bp	1.0	55.8
trnS-GCU-trnG-GCC_p2	TTTAGTCCCCTCAGCCATCT	ACGAATCACACTTTTACCACT	50.0	38.1	57.7	55.5	799-823 bp	1.0	55.3
trnS-GCU-trnG-GCC_p3	CGGAGAGAGAGGGATTCGAA	ACGAATCACACTTTTACCACT	55.0	38.1	58.3	55.5	863-887 bp	1.0	54.8
trnS-GCU-trnG-GCC_p4	TTAGTCCCCTCAGCCATCTC	AGAACGAATCACACTTTTACCAC	55.0	39.1	58.2	57.3	801-825 bp	1.0	54.8
trnS-GCU-trnG-GCC_p5	TTAGTCCCCTCAGCCATCTC	GAACGAATCACACTTTTACCACT	55.0	39.1	58.2	57.3	800-824 bp	1.0	54.8