Hotspot

rps16-trnQ-UUG

LSC · 6115-7252 · 1138 bp

Back to Cephalanthera

Pi 0.0263

MarkerSeek score 73.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1138
variable_sites: 72
pi: 0.0263
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	ATCCACAGCCAATCATGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1015-1242 bp	1.0	87.7
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	992-1219 bp	1.0	87.2
rps16-trnQ-UUG_p3	CCACAGCCAATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1013-1240 bp	1.0	87.0
rps16-trnQ-UUG_p4	ATCCACAGCCAATCATGTCC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	57.9	57.8	1019-1246 bp	1.0	86.6
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1003-1230 bp	1.0	86.6