Hotspot

trnN-GUU-trnR-ACG

IRa · 129982-130290 · 309 bp

Back to Centrosema

Pi 0.0194
MarkerSeek score 63.90
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
309
variable_sites
6
pi
0.0194
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 CCGTTGTTCCTCAGTAGCTC GGTACTCCGGGGAACAGATA 55.0 55.0 58.0 57.9 503-510 bp 1.0 80.3
trnN-GUU-trnR-ACG_p2 TGGGTTGAGAACGGGAATTG GGTACTCCGGGGAACAGATA 50.0 55.0 58.1 57.9 544-551 bp 1.0 79.5
trnN-GUU-trnR-ACG_p3 CCGTTGTTCCTCAGTAGCTC CAGAGGATTAGAGCACGTGG 55.0 55.0 58.0 57.8 450-457 bp 1.0 79.3
trnN-GUU-trnR-ACG_p4 GCTGTTAACTGACTGGTCGT GGTACTCCGGGGAACAGATA 50.0 55.0 57.8 57.9 467-474 bp 1.0 79.2
trnN-GUU-trnR-ACG_p5 TGGTCGTAGGTTCGAATCCT GGTACTCCGGGGAACAGATA 50.0 55.0 58.2 57.9 454-461 bp 1.0 79.2