Hotspot

rpl32-trnL-UAG

SSC · 113622-114169 · 548 bp

Back to Centrosema

Pi 0.0307
MarkerSeek score 72.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
548
variable_sites
16
pi
0.0307
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCGAAATCCATTTTTACAGGGA TCGAACCGAGATGCTTTAGC 40.9 50.0 57.3 58.1 662-673 bp 1.0 62.7
rpl32-trnL-UAG_p2 AGCGAAATCCATTTTTACAGGG TCGAACCGAGATGCTTTAGC 40.9 50.0 57.3 58.1 663-674 bp 1.0 62.7
rpl32-trnL-UAG_p3 GCGAAATCCATTTTTACAGGGA TCCTAAGAGCAGCGTGTCTA 40.9 50.0 57.3 57.9 636-647 bp 1.0 62.5
rpl32-trnL-UAG_p4 AGCGAAATCCATTTTTACAGGG TCCTAAGAGCAGCGTGTCTA 40.9 50.0 57.3 57.9 637-648 bp 1.0 62.5
rpl32-trnL-UAG_p5 GCGAAATCCATTTTTACAGGGA AGCAGCGTGTCTACCAATTT 40.9 45.0 57.3 57.8 629-640 bp 1.0 62.1